My research interests are focused on biological molecular motors. I study myosin II, the protein responsible for force generation in muscle, cell locomotion and intracellular trafficking. I combine protein mutagenesis, site directed labeling, multifrequency Electron Paramagnetic Resonance (EPR) and transient time-resolved fluorescence resonance energy transfer (TR2FRET) to explore myosin functional dynamics on molecular and submolecular level. The ultimate goal of these studies is to determine the mechanism of force generation in myosin.
I am also interested in manipulating of microwave field distribution in EPR cavity to maximize sensitivity of EPR measurement for aqueous samples, through the sample geometry optimization and usage of ferroelectric/dielectric cavity inserts.
Please visit NesmelovLab web page for more details